Greek Genome Center
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery. Among others, DNA sequencing is used for identifying:
The complete DNA sequence of an organism’s genome (Whole Genome Seq, WGS)
The precise DNA sequencing of all the protein-coding genes in a genome (Whole Exome Seq, WES) or of a specific group of genes (Gene Panel Seq)
The binding sites of a DNA-associated protein (ChIP-seq)
The DNA regions in the genome associated with regulatory activity (DNAse-seq, FAIRE-seq)
The sequences that act as transcriptional enhancers (STARR-seq)
A high resolution tool for studying changes in the coding and noncoding regions of the transcriptome. RNA-Seq enables the sensitive and accurate measurement of differential gene expression over time or in different groups, conditions and treatments. RNA-Seq also enables the detection of transcript isoforms, gene fusions, single nucleotide variants (SNVs) and allele-specific gene expression.